Capture Sequencing to Explore and Map Rare Casein Variants in Goats

Genetic variations in the four casein genes CSN1S1 , CSN2 CSN1S2 and CSN3 have obtained substantial attention since they affect milk protein yield, composition, cheese processing properties, digestibility as well tolerance human nutrition. Furthermore, variants are used for breed characterization, biodiversity, phylogenetic studies. The current study aimed at identification of five domestic goat breeds from Sudan (Nubian, Desert, Nilotic, Taggar, Saanen) three wild species [ Capra aegagrus (Bezoar ibex), nubiana (Nubian ibex (Alpine ibex)]. High-density capture sequencing 33 goats identified total 22 non-synonymous 13 synonymous single nucleotide polymorphisms (SNPs), which nine seven SNPs new. In gene, new SNP ss7213522403 segregated Alpine ibex. ss7213522526, ss7213522558, ss7213522487 were found exclusively Nubian ss7213522477, ss7213522549, ss7213522575 only. ss7213522604 ss7213522610 DNA sequence led to detection variants. New detected alpha S1 Saanen ( ∗ C1), Bezoar J), K), beta kappa caseins F X), S2 all domesticated H), Desert I), or only J K). results show that most novel occur critically endangered This highlights importance preservation this breed. we suggest validating further characterizing